Clinical Implementation of Comprehending Dentin on a Microscopic Level

The aim of the present in vitro study is to visualize dentin get an in-depth knowledge nature that could provide useful information regarding conditioning dentinal substrate when treating lesions. Forty-nine extracted human third molars were obtained and prepared produce artificial lesions through demineralizing with acetic acid for 7 14 days, or lactic days. teeth divided into groups treated either NaOCl, pepsin, trypsin, phosphoric acid. To obtain on morphology surfaces, all samples visualized under high resolution field emission scanning electron microscope. With magnification reaching x50000 was clearly together its constitutes. effect various demineralization approaches treatment protocols demonstrated clearly. relationship between procedure steps subsequent bond strength discussed. our best knowledge, there no previous clear highly magnified microscope images dentin, components constitutes without treatments. current suggests complexity as a should be carefully especially technique sensitive procedures such adhesive restorations.